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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression analysis of Heat Shock in Drosophila melanogaster KC167 Cells and 3rd instar dpcnbw larvae

ABSTRACT: Expression analysis of Heat Shock in Drosophila melanogaster KC167 Cells and 3rd instar dpcnbw larvae, and room temperature expression levels using Nimblegen arrrays (GPL8443) A 12 chip study using total RNA recovered from Heat shocked or non-heat shocked (room temperature) samples in either cells or larvae, 3 technical replicates for each treatment

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Zak Razak

PROVIDER: E-GEOD-19745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis during heat shock in Drosophila melanogaster KC167 cells and 3rd instar dp cn bw larvae

Project description:Gene expression analysis of Drosophila melanogaster Kc167 cells and 3rd instar dp cn bw larvae, under heat shock and non-heat shock conditions (room temperature) using Nimblegen arrrays (GPL8443)

2011-01-05 | GSE19745 | GEO

Gene expression analysis during heat shock in Drosophila melanogaster 3rd instar hsf4 cn bw larvae

Project description:Gene expression analysis of Drosophila melanogaster 3rd instar hsf4 cn bw larvae, under heat shock and non-heat shock conditions (room temperature) using Nimblegen arrrays (GPL8443)

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ecdysone treated KC167 cells during Heat Shock and at Room Temperature

Project description:ecdysone treated KC167 cells during Heat Shock and at Room Temperature

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Transcriptome analysis of heat stressed Nicotiana tabacum pollen tubes

Project description:Along with lipidomic and metabolomic analyses, we analysed the effect of short-term heat stress on Nicotiana tabacum pollen tubes. Tubes were either grown for 3 hours at room temperature, for 6 hours at room temperature or for 3 hours at room temperature and then 37 °C for another 3 hours.

2021-06-26 | GSE153474 | GEO

Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster at room temperature

Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA from non-shock (room temperature) Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.

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Small RNA-seq during the ecdysone and heat-shock response in Drosophila Kc167 cells

Project description:Small RNA sequencing libraries at 0 h, 3 h, and 48 h time points after ecdysone treatment and 20min heat shock (37 degrees celcius) in Drosophila cell culture Examination of small RNA dynamics during differentiation

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Transcriptional profiling of Caenorhabditis elegans and Caenorhabditis briggsae before and after heat shock

Project description:Transcriptional profiling of heat-shocked worms was compared to non-heat-shocked worms to determine genes that are induced upon heat shock in each species; heat-shock-induced genes in each species were compared

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Molecular characterization of the apical organ in the anthozoan Nematostella vectensis

Project description:The aim of the experiment was the identification of genes that are expressed in the apical organ of the sea anemone Nematostella vectensis. To achieve this, animals were generated that either lack the apical organ (injected with a morpholino antisense oligonucleotide to block the function of NvFGFa1) or have an expanded apical organ (injected with a morpholino antisense oligonucleotide to block the function of NvFGFa2, see Rentzsch et al., Development, 2008). Eggs from the same batch were injected after fertilization, (MO NvFGFa1 at 0.5uM, MO NvFGFa2 at 0.25uM) and planula larvae with the expected phenotype and without general developmental abnormalities were selected after 72 hours of development at room temperature (22°C), and placed in RNAlater Solution (Ambion) for subsequent extraction of RNA. Three conditions were isolated: embryos injected with NvFGFa1 MO, embryos injected with NvFGFa2 MO and non-injected embryos. Total RNA was extracted with the RNAqueous kit (Ambion), following the manufacturer’s protocol, and temporarily stored at -80°C, in order to accumulate the quantity necessary for the hybridization. The isolated total RNA for each condition was pooled and split into to two tubes and shipped on dry ice to Nimblegen in Iceland. The injections and the isolation of total RNA were performed from May – August 2009 at the Sars Centre in Bergen, Norway.

2014-10-19 | E-MTAB-3004 | biostudies-arrayexpress

Genome-wide expression analysis of dikaryotic colonies of Schizophyllum commune under various conditions: wild-type, delta-wc2, delta-wc2 hsp3(pr)-wc2

Project description:The blue light receptor WC-2 was shown to be involved in mushroom development in the basidiomycete Schizophyllum commune. When the gene encoding WC-2 was deleted, no mushrooms formed and colony morphology was radial. This phenotype was similar to the wild-type colony grown in the dark. This phenotype could be complemented by transforming the wc-2 deletion strain with a construct encompassing the wc-2 coding sequence under the control of the heat inducible promoter hsp3. A daily heat shock of 1 hour at 42 degrees Celsius resulted in mushroom development and an asymmetrical colony. In this study we performed a genome-wide expression analysis on dikaryons of wild-type (not heat shocked), delta-wc2 (heat shocked or not heat shocked) and the complemented strain delta-wc2 hsp3-wc2 (heat shocked or not heat shocked).

2012-09-24 | GSE24007 | GEO

Thermoprotection in the Drosophila hsf4 mutant

Project description:In Drosophila larvae, acquired synaptic thermotolerance following heat shock has previously been shown to correlate with the induction of heat shock proteins (Hsps) including HSP70. We tested the hypothesis that synaptic thermotolerance would be significantly diminished in a temperature-sensitive strain (hsf4) which has been reported not to be able to produce inducible Hsps in response to heat shock. Contrary to our hypothesis, considerable thermoprotection was still observed at hsf4 larval synapses following heat shock. To investigate the cause of this thermoprotection, we conducted DNA microarray experiments to identify heat-induced transcript changes in these organisms. Transcripts of the hsp83, dnaJ-1(hsp40) and gstE1 genes were significantly up-regulated in hsf4 larvae after heat shock. In addition, increases in the levels of Hsp83 and DnaJ-1 proteins but not in the inducible form of Hsp70 were detected by Western blotting. The mode of heat shock administration differentially affected the relative transcript and translational changes for these chaperones. These results indicate that the compensatory up-regulation of constitutively expressed Hsps, in the absence of the synthesis of inducible Hsps including HSP70, could still provide substantial thermoprotection to both synapses and the whole organism. Two strains were used in this study; the mutant cn bw hsf4 strain, and the dp cn bw cl control strain from which the mutant was derived. For each strain, co-reared larvae were separated into a control group and a treatment group at random. RNA from the heat-shocked larvae and from the respective controls were directly compared on cDNA microarrays (GPL311). Two independent biological samples from each genotype were collected. In addition, an inter-strain comparison was made using the RNA from the untreated controls from each genotype. This was also performed in duplicate.

2005-11-30 | E-GEOD-2998 | biostudies-arrayexpress

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