Transcription profiling and genotyping, detection of allele specific gene expression of Saccharomyces BY, isogenic to S288C, and RM, a wild vineyard strain

ABSTRACT: By hybridizing mRNA to oligonucleotide arrays and searching for probes that are outliers in their probe set, we identify and genotype polymorhisms in two strains of yeast (BY, isogenic to S288C, and RM, a wild vineyard strain) and segregants from a cross between the two strains. We then use this mRNA based genotyping approach to study allele-specific expression in diploid hybrids from a cross between BY and RM. A 1:1 mixture of BY and RM parental mRNA is created and hybridized to arrays as a control in these allele-specific expression experiments. The S. cerevisiae strains BY4716, an S288C derivative, and RM11-1a, a haploid Bb32(3) derivative, are described elsewhere (Brem et al. 2002, Yvert et al. 2003). We grew cultures to 10e7 cells/mL in shake flasks at 175 rpm at 300 C in syntheti

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: James Ronald 

PROVIDER: E-GEOD-1975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress