Gene expression effects of morpholino directed spRunt-1 knockdown on hatched blastula stage embryos

ABSTRACT: Runt domain (Runx) transcription factors constitute a metazoan family of sequence specific DNA binding proteins that are essential for animal development. The sea urchin Runx gene SpRunt-1, which is expressed globally during early embryogenesis, is required for blastula stage cell proliferation and subsequently for cell survival. To obtain a comprehensive list of SpRunt-1 regulatory targets we screened a custom microarray representing the blastula stage transcriptome of Strongylocentrotus purpuratus, comparing relative hybridization signals generated by labeled RNA from 18 hr control versus SpRunt-1 morphant embryos. For an 8x15k chip the 8 arrays were 4 biological replicates and a dye swap of the same 4. Biological Replicates - Four separate embryo cultures, each from a differe

ORGANISM(S): Strongylocentrotus purpuratus

SUBMITTER: Anthony Robertson 

PROVIDER: E-GEOD-19751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress