L-Glutamine metabolism and its regulation in C. glutamicum

ABSTRACT: Corynebacterium glutamicum, a gram-positive soil bacterium used for the industrial production of amino acids such as L-glutamate and L-lysine, is able to use a number of different nitrogen sources, such as ammonium, urea, or creatinine. In this communication, we show that L-glutamine serves as an excellent nitrogen source for C. glutamicum and allows similar growth rates in glucose minimal medium as ammonium. A transcriptome comparison revealed a strong induction of the nitrogen starvation response when glutamine was used as nitrogen source. Subsequent growth experiments with a variety of mutants defective in nitrogen metabolism showed that glutamate synthase is crucial for glutamine utilization, while a putative glutaminase is dispensable under the experimental conditions used. The fact t

ORGANISM(S): Corynebacterium glutamicum ATCC 13032

SUBMITTER: Andreas Burkovski 

PROVIDER: E-GEOD-19779 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress