Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of hexachlorocyclohexane (HCH) contaminated soils from Spain with a community-specific microarray


ABSTRACT: Comparison of hexachlorocyclohexane (HCH) contaminated soils from Spain with a community-specific microarray. These results are being submitted for publication and represent the first use of microarrays for analysis of soil DNA and the first community-specific microarray design. Keywords: other

ORGANISM(S): Bacteria

SUBMITTER: William Mohn 

PROVIDER: E-GEOD-1985 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Composition of microbial communities in hexachlorocyclohexane (HCH) contaminated soils from Spain revealed with a habitat-specific microarray.

Neufeld Josh D JD   Mohn William W WW   de Lorenzo Victor V  

Environmental microbiology 20060101 1


Microarray technology was used to characterize and compare hexachlorocyclohexane (HCH) contaminated soils from Spain. A library of 2,290 hypervariable 16S rRNA gene sequences was prepared with serial analysis of ribosomal sequence tags (SARST) from a composite of contaminated and uncontaminated soils. By designing hybridization probes specific to the 100 most abundant ribosomal sequence tags (RSTs) in the composite library, the RST array was designed to be habitat-specific and predicted to monit  ...[more]

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