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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Drosophila melanogaster taste tissue

ABSTRACT: To uncover novel molecules involved in taste detection, we performed a microarray-based screen for genes enriched in taste neurons. Proboscis RNA from flies homozygous for a recessive poxn null mutation was compared to RNA from heterozygous controls. Poxn mutants have a transformation of labellar gustatory chemosensory bristles into mechanosensory bristles and therefore lack most or all taste neurons. Experiment Overall Design: Proboscises of poxn70 homozygous mutant and poxn70 heterozygous mutant males (8-18 days post eclosure) were dissected, and total RNA was harvested in Trizol according to standard trizol protocol. Samples for each microarray were prepared from 164-280 proboscises. We performed 3 biological replicates for each genotype.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Peter Cameron

PROVIDER: E-GEOD-19984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The molecular basis for water taste in Drosophila.

Cameron Peter P Hiroi Makoto M Ngai John J Scott Kristin K

Nature 20100404 7294

The detection of water and the regulation of water intake are essential for animals to maintain proper osmotic homeostasis. Drosophila and other insects have gustatory sensory neurons that mediate the recognition of external water sources, but little is known about the underlying molecular mechanism for water taste detection. Here we identify a member of the degenerin/epithelial sodium channel family, PPK28, as an osmosensitive ion channel that mediates the cellular and behavioural response to w ...[more]

PMID: 20364123

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