Evolutionarily conserved replication timing profiles distinguish cell types and predict long range chromatin interaction

ABSTRACT: Extensive changes in replication timing occur during early mouse development, but their biological significance remains uncertain. To identify evolutionarily conserved features of replication timing and their relationships to epigenetic properties in humans, we profiled replication timing genome-wide in four human embryonic stem cell (hESC) lines, hESC-derived neural precursor cells (NPCs), lymphoblastoid cells, and two independently derived human induced pluripotent stem cell lines (hiPSCs). Results confirm the conservation of coordinately replicated megabase-sized units of chromosomes (replication domains) with stable cell type specific molecular boundaries that consolidate into larger replication domains during differentiation. Replication timing changes encompassed units of 400-800 kb

ORGANISM(S): Homo sapiens

SUBMITTER: Ichiro Hiratani 

PROVIDER: E-GEOD-20027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress