Generation of Autologous Pluripotent Stem Cells from Adult Testis Biopsy

ABSTRACT: In regenerative medicine, histocompatibility of pluripotent stem cells is required to solve the problem of immunorejection after therapeutic transplantation. In this study, we show that autologous germline stem cells (GSCs), often called spermatogonial stem cells, could be derived by testis biopsy from individual mice and that GSCs subsequently could be dedifferentiated into autologous germline-derived pluripotent stem (gPS) cells. The establishment of GSCs by testicular biopsy in the mouse model can prove the principle of human clinical application to derive autologous GSCs to generate patient-specific pluripotent cells for regenerative medicine. For transcriptome profiling, 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Ki

ORGANISM(S): Mus musculus

SUBMITTER: Marcos Arauzo-Bravo 

PROVIDER: E-GEOD-20031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress