Vinyl Chloride Degradation by Mixed Microbial Community KB1

ABSTRACT: A shotgun metagenome microarray was created and used to investigate gene transcription during vinyl chloride (VC) dechlorination by a microbial enrichment culture called KB1. The array was constructed by spotting genomic fragments amplified from short-insert libraries of KB1 metagenomic DNA. Subsequently, the microarrays were interrogated with RNA extracted from KB1 during VC dechlorination (VC+methanol), and in the absence of VC (methanol-only). The most differentially expressed spots, and spots with the highest intensities, were then chosen to be sequenced. Sequencing revealed that Dehalococcoides (Dhc) genes involved in transcription, translation and energy generation were up-regulated during VC degradation. Furthermore, the results indicated that the reductive dehalogenase homolog

ORGANISM(S): dechlorinating enrichment culture KB-1

SUBMITTER: Alison Waller 

PROVIDER: E-GEOD-20041 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress