Temporal gene expression by Ephedra herba in brain, heart and liver of mouse
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Bu-Yeo Kim
PROVIDER: E-GEOD-20084 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:A microarray analysis was conducted using the brain tissue of mouse orally administrated aqueous extracts of Ephedra herba, in which the expression level of many genes were altered depending on the duration of treatment time. In particular, temporal changes in the expressions of genes can be classified into two major reciprocal patterns. Aqueous extracts of Ephedra herba were administered orally at dosage of 50 or 1,250 mg/kg once daily for 1, 3 and 10 consecutive days on mice. Expression level of genes from brain tissue was compared to that from untreated control mice.
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Project description:A microarray analysis was conducted using the brain, heart, and liver tissues of mouse orally administrated aqueous extracts of Ephedra herba, in which the expression level of many genes were altered depending on the duration of treatment time.
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Project description:A microarray analysis was conducted using the brain tissue of mouse orally administrated aqueous extracts of Ephedra herba, in which the expression level of many genes were altered depending on the duration of treatment time. In particular, temporal changes in the expressions of genes can be classified into two major reciprocal patterns.
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Project description:We have recently developed a Drosophila behavioral- and transcriptomic- based (systems) model of chronic pentylenetetrazole (PTZ) induced locomotor plasticity. Pharmacological validation using antiepileptic drugs (AEDs) sodium valproate and levetiracetam (LEV) at behavioral level showed a weaker prophylactic and stronger symptomatic effect of sodium valproate and the opposite, i.e., stronger prophylactic and weaker symptomatic, effect in the model. Microarray gene expression profiling of fly head after sodium valproate and LEV treatment of flies further supported the above therapeutic effect of the two AEDs in the PTZ fly model. Given the strong prophylactic effect of LEV in our model, we hypothesized that time series of transcriptomic alterations caused by PTZ at 12 hrs, 2nd day and 7th day (an earlier GEO submission) may be neutralized by LEV. 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