Retinal pigment epithelium and photoreceptor and choroidal cells

ABSTRACT: Background: To identify and functionally annotate cell type-specific gene expression in the human retinal pigment epithelium (RPE), a key tissue involved in age-related macular degeneration and retinitis pigmentosa. Methodology: RPE, photoreceptor and choroidal cells were isolated from selected freshly frozen healthy human donor eyes using laser microdissection. RNA isolation, amplification and hybridization to 44k microarrays was carried out according to Agilent specifications. Bioinformatics was carried out using Rosetta Resolver, David and Ingenuity software. Principal Findings: Our previous 22k analysis of the RPE transcriptome showed that the RPE has high levels of protein synthesis, strong energy demands, is exposed to high levels of oxidative stress and a variable degree of inflamma

ORGANISM(S): Homo sapiens

SUBMITTER: Judith Booij 

PROVIDER: E-GEOD-20191 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress