Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of rat cardiac myocytes and fibroblasts subjected to 24 hours of mechanical stress, or treated with a single dose of ionizing or ultraviolet radiation and allowed to recover for 24 hours

ABSTRACT: Rationale: During excessive pressure or overload, cardiac cells are subjected to increased mechanical stress. We investigate how the stress response of cardiac cells to mechanical stress can be compared to genotoxic stresses induced by DNA damaging agents. Methods: Cultures enriched for cardiomyocytes and cultures of fibroblasts were derived from ventricles of neonatal rat hearts. In three independent experiments, both cell cultures were subjected to mechanical stress (cyclic stretch at 60 cycles/min), UV irradiation (10 J/m2), or X-rays (8.5 Gy). Cells were harvested 24 h. after (the onset of) treatment. Total RNA was isolated using an RNeasyÂ® kit (Qiagen). Single stranded cDNA was synthesized from RNA from myocyte-enriched populations (10-14 microgram) and populations of fibroblasts (7.5-14 microgram), using Superscript II reverse transcriptase and T7-oligo(dT)24 primers at 42ÂºC for 1 h. Double stranded cDNA was obtained by using DNA ligase, DNA polymerase I and RNAse H at 16ÂºC for 2 h, followed by T4 DNA polymerase at 16ÂºC for 5 min. cRNA was synthesized from cDNA using the BioArray HighYieldÂ® RNA transcript labeling kit (Enzo) in the presence of biotin-labeled CTP and UTP. cRNA was fragmented in a buffer containing 40 mM Tris-acetate (pH 8.1), 100 mM KAc and 30 mM MgAc, at 94ÂºC for 35 min. Fragmented cRNA was hybridized to GeneChip RG-U34A arrays (Affymetrix) at 45ÂºC for 16 h. Arrays were washed and incubated with a streptavidin-phycoerythrin complex. Fluorescent signals were determined with a GeneChip scanner.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Marjan Boerma

PROVIDER: E-GEOD-2032 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Microarray analysis of gene expression profiles of cardiac myocytes and fibroblasts after mechanical stress, ionising or ultraviolet radiation.

Boerma Marjan M van der Wees Caroline G C CG Vrieling Harry H Svensson J Peter JP Wondergem Jan J van der Laarse Arnoud A Mullenders Leon H F LH van Zeeland Albert A AA

BMC genomics 20050118

<h4>Background</h4>During excessive pressure or volume overload, cardiac cells are subjected to increased mechanical stress (MS). We set out to investigate how the stress response of cardiac cells to MS can be compared to genotoxic stresses induced by DNA damaging agents. We chose for this purpose to use ionising radiation (IR), which during mediastinal radiotherapy can result in cardiac tissue remodelling and diminished heart function, and ultraviolet radiation (UV) that in contrast to IR induc ...[more]

PMID: 15656902

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