Transcription profiling of rat cardiac myocytes and fibroblasts subjected to 24 hours of mechanical stress, or treated with a single dose of ionizing or ultraviolet radiation and allowed to recover for 24 hours

ABSTRACT: Rationale: During excessive pressure or overload, cardiac cells are subjected to increased mechanical stress. We investigate how the stress response of cardiac cells to mechanical stress can be compared to genotoxic stresses induced by DNA damaging agents. Methods: Cultures enriched for cardiomyocytes and cultures of fibroblasts were derived from ventricles of neonatal rat hearts. In three independent experiments, both cell cultures were subjected to mechanical stress (cyclic stretch at 60 cycles/min), UV irradiation (10 J/m2), or X-rays (8.5 Gy). Cells were harvested 24 h. after (the onset of) treatment. Total RNA was isolated using an RNeasy® kit (Qiagen). Single stranded cDNA was synthesized from RNA from myocyte-enriched populations (10-14 microgram) and populations of fibroblasts (7.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Marjan Boerma 

PROVIDER: E-GEOD-2032 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress