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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression analysis data from large T antigen-immortalized murine embryonic fibroblasts (MEFs)

ABSTRACT: Microarrays were used to examine the genome-wide expression in FIH null, VHL null and VHL/FIH double null MEFs. We used these data to analyze how deletion of FIH or VHL alone affects gene expression and if VHL and FIH have synergistic effects and differential selectivity on regulating gene expression. To assess how deletion of FIH, VHL and both VHL and FIH affect gene expression genome-widely, we generated FIH null, VHL null and VHL/FIH double null MEFs after adeno-cre virus infection on large T-immortalized FIHdf, VHLdf, and VHLdf/FIHdf MEFs. These MEFs were cultured under normoxia (21% O2) with complete culture medium before RNA extraction. Total RNA were isolated by using Qiagen RNeasy kit and treated with on-column DNase digestion. Affymetrix GeneChip Mouse Genome 430 2.0 Array was used.

ORGANISM(S): Mus musculus

SUBMITTER: Na Zhang

PROVIDER: E-GEOD-20335 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression analysis data from large T antigen-immortalized murine embryonic fibroblasts (MEFs)

Project description:Microarrays were used to examine the genome-wide expression in FIH null, VHL null and VHL/FIH double null MEFs. We used these data to analyze how deletion of FIH or VHL alone affects gene expression and if VHL and FIH have synergistic effects and differential selectivity on regulating gene expression.

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PCAF/GCN5-regulated genes in MEFs

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2013-12-31 | E-GEOD-20008 | biostudies-arrayexpress

Gene expression analysis in primary human renal tumors, categorized by VHL genotype and HIF-alpha expression

Project description:Human renal cell carcinomas (RCC) have differential expression of HIF-1alpha and HIF-2alpha, depending on VHL genotype and other events. Here, we have divided a series of RCC samples for HIF-alpha expression and VHL genotype, in order to define differentially expressed genes Keywords: Patient Sample Study A total of 57 frozen RCC samples were stained for HIF-1alpha and HIF-2alpha, and genotyped for VHL. 5 VHL WT/HIF-negative, 8 VHL-deficient/HIF-1alpha+/HIF-2alpha+ and 8 VHL-deficient/HIF-2alpha+ tumors were selected for microarray

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Vhl and Pbrm1 double deletion effect on kidney

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Primary murine embryonic fibroblasts (MEFs): Cdk7 wild type versus Cdk7 KO

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Expression data for Lck-Cre, Med23flox/flox and Med23flox/flox;Lck-Cre thymocytes +/- 3hr exposure to plate bound anti-CD3 antibody

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2014-07-30 | E-GEOD-57061 | biostudies-arrayexpress

Transcriptomic output of endogenous KRasG12D and modestly overexpressed MYC in MEFs

Project description:We sought to compare the transcriptomic output of Kras activation with that of modestly overexpressed cMYC, to better understand the mechanism of oncogenic cooperation. We isolated fibrobalsts from mouse embryos carrying CRE-inducible alleles for KRasG12D (LSL-KRasG12D) and MYC (Rosa26-LSL-MYC), alongside double positive and wild-type control MEFs. All MEFs were infected at early passage with Adeno-CMV-CRE, to induced expression of conditional alleles, where present, and RNA was isolated 24hrs post infection.

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Mus musculus

Project description:Identification of transcriptional changes upon genetic deletion of Vhl, Pbrm1 and double deletion of both Vhl and Pbrm1

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Primary murine embryonic fibroblasts (MEFs): Cdk7 wild type versus Cdk7 KO

2012-04-18 | GSE36050 | GEO

Expression Data from wild type and E2F4 null MEFs

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2008-07-11 | GSE11039 | GEO

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