Profiling the transcriptional response upon deletion of PUN1

ABSTRACT: Yeast filamentous growth is a stress response to conditions of nitrogen deprivation, wherein yeast colonies form pseudohyphal filaments of elongated and connected cells. As proteins mediating adhesion and transport are required for this growth transition, the protein complement at the yeast cell periphery plays a critical and tightly regulated role in enabling pseudohyphal filamentation. To identify proteins differentially abundant at the yeast cell periphery during pseudohyphal growth, we generated quantitative proteomic profiles of plasma membrane protein preparations under conditions of vegetative growth and filamentation. By iTRAQ chemistry and two-dimensional LC-MS/MS, we profiled 2,463 peptides and 356 proteins, from which we identified eleven differentially abundant proteins that

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Tao Xu 

PROVIDER: E-GEOD-20351 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress