Transcription profiling of E. coli cultures grown on different carbon sources

ABSTRACT: The study investigated the effect of different carbon sources on E. coli global gene expression. We grew MG1655 cells aerobically in MOPS minimal medium with either glucose, glycerol, succinate, L-alanine, acetate, or L-proline as the carbon supply. Samples were taken from each culture at mid log phase and were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent (Qiagen). Total RNA was then isolated using MasterPure kits (Epicentre Technologies). Purified RNA was reverse-transcribed to cDNA, labeled and hybridized to Affymetrix GeneChipÒ E. coli Antisense Genome Arrays as recommended in the technical manual (www.affymetrix.com).

ORGANISM(S): Escherichia coli

SUBMITTER: Mingzhu Liu 

PROVIDER: E-GEOD-2037 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress