A large fraction of extragenic RNA Pol II transcription sites overlap enhancers

ABSTRACT: Mammalian genomes are pervasively transcribed outside mapped protein-coding genes. One class of extragenic transcription products is represented by long non-coding RNAs (lncRNAs), some of which result from Pol_II transcription of bona-fide RNA genes. Whether all lncRNAs described insofar are products of RNA genes, however, is still unclear. Here we have characterized transcription sites located outside protein-coding genes in a highly regulated response, macrophage activation by endotoxin. Using chromatin signatures, we could unambiguously classify extragenic Pol_II transcription sites as belonging to either canonical RNA genes or transcribed enhancers. Unexpectedly, 70% of extragenic Pol_II peaks were associated with genomic regions with a canonical chromatin signature of enhancers. Enhan

ORGANISM(S): Mus musculus

SUBMITTER: Iros Barozzi 

PROVIDER: E-GEOD-20370 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress