Metabolic and transcriptional response to cofactor perturbations in Escherichia coli

ABSTRACT: Metabolic cofactors such as NADH and ATP play important roles in a large number of cellular reactions and it is of great interest to dissect the role of these cofactors in different aspects of metabolism. Towards this goal, we overexpressed NADH oxidase and the soluble F1-ATPase in Escherichia coli to lower the level of NADH and ATP, respectively. We used a systems biology approach to study the response to these perturbations by measuring global transcription profiles, metabolic fluxes and the metabolite levels. We integrated information from the different measurements using network-based methods to identify high-scoring networks in a global interaction map that included protein interactions, transcriptional regulation and metabolism. The results revealed that the action of many global tra

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Goutham Vemuri 

PROVIDER: E-GEOD-20374 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress