LSEC: a microenvironment dependent differentiation program in rat including the novel junctional protein Leda-1

ABSTRACT: Liver sinusoidal endothelium (LSEC) is a prime example for organ-specific microvascular differentiation and functions. Disease-associated capillarization of LSEC in vivo and dedifferentiation of LSEC in vitro indicate the importance of the hepatic microenvironment. To identify the LSEC-specific molecular differentiation program in the rat, we used a two-sided gene expression profiling approach comparing LSEC freshly isolated ex vivo with both lung microvascular endothelial cells (LMEC) and with LSEC cultured for 42h. The LSEC signature consisted of 48 genes both down-regulated in LMEC and in LSEC upon culture (FC>7 in at least one comparison); qRT-PCR confirmation of these genes included numerous family members and signalling pathway-associated molecules. The LSEC differentiation program c

ORGANISM(S): Rattus norvegicus

SUBMITTER: Kai Schledzewski 

PROVIDER: E-GEOD-20375 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress