Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PTC1 mutant Yeast Genomic Expression Patterns in Response to deoxynivalenol

ABSTRACT: The evaluation of the toxicity of deoxynivalenol using yeast gene expressions. Yeast PTC1 mutant strain was used for this study. SDS was used to raise the penetration of the yeast cell membrane. Keywords: stress response Two conditions were compared by the gene expression profiles. Both samples were grown in 0.01% SDS contained YPD media, and one of them was also supplied with 50 ppm of deoxynivalenol.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Tadahiro Suzuki

PROVIDER: E-GEOD-20378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Yeast genomic expression patterns in response to deoxynivalenol

Project description:The assessment of toxicity about deoxynivalenol (DON) using yeast gene expression comparison analysis. Two yeast strains, parental BY4743 and PTC1 mutant, were used for this study. SDS was used to raise the penetration of the yeast cell membrane. Keywords: stress response Four conditions are compared with three replicates each. Each of the two strains was grown in either 0.01% SDS-containing YPD media or 0.01% SDS-containing YPD media with 25 ppm of deoxynivalenol.

2010-06-08 | E-GEOD-14748 | biostudies-arrayexpress

PTC1 mutant Yeast Genomic Expression Patterns in Response to deoxynivalenol

Project description:The evaluation of the toxicity of deoxynivalenol using yeast gene expressions. Yeast PTC1 mutant strain was used for this study. SDS was used to raise the penetration of the yeast cell membrane. Keywords: stress response

2011-07-22 | GSE20378 | GEO

Yeast Genomic Expression Patterns of Wild-type and PTC1 null mutant in Response to Aflatoxin B1 (AFB1)

Project description:The evaluation of the toxicity of Aflatoxin B1 using yeast gene expressions. Two yeast strains; parental BY4743 and PTC1 mutant, were used for this study. SDS was used to raise the penetration of the yeast cell membrane. Two conditions are compared with three replicates each. Both strains were grown in 0.01% SDS containing YPD media or 0.01% SDS, 25 ppm ABF1 containing YPD media. Publication can be found at http://www.cbi.or.jp/cbi/CBIj/vol9/9_94-E.pdf.

2010-04-12 | E-GEOD-17367 | biostudies-arrayexpress

Yeast genomic expression patterns in response to deoxynivalenol

2010-05-31 | GSE14748 | GEO

Yeast Genomic Expression Patterns of PTC1 null mutant in Response to Aflatoxin B1 (AFB1)

Project description:The evaluation of the toxicity of Aflatoxin B1 using yeast gene expressions. Yeast strain; BY4743 PTC1 mutant, was used for this study. SDS was used to raise the penetration of the yeast cell membrane.

2010-04-01 | GSE17367 | GEO

Yeast Genomic Expression Patterns in Response to glucosylated mycotoxin

Project description:The evaluation of mycotoxicity about glucosylated mycotoxin using yeast gene expression comparison analysis. Yeast BY4743 derivative PDR5 mutant was used for this study. Samples were normalized with the three replicates per condition. Yeast strain was grown in YPD medium with 80 Î¼M of deoxynivalenol-3-glucoside. The same volume of DMSO was added into control samples.

2015-04-01 | E-GEOD-63663 | biostudies-arrayexpress

Salmonella Typhimurium: control vs. Deoxynivalenol (DON) treatment

Project description:Transcriptional profiling of log and stationary phase S. Typhimurium, comparing untreated controls with Deoxynivalenol treated samples. Each array used labelled cDNA against a common genomic DNA reference. Triplicate arrays were carried out for each of the 4 conditions: untreated log phase, untreated stationary phase, DON treated log phase and DON treated stationary phase

2011-05-20 | E-GEOD-29399 | biostudies-arrayexpress

Transcriptional profiling of Rapamycin treatment in yeast cells lacking the Ptc1 protein phosphatase.

Project description:We are interested in the study of the already reported sensitivity of yeast cells lacking the type 1 protein phosphatase Ptc1 to the drug rapamycin. In order to carry out our studies, we analyzed the changes in the transcription profile that a short-term incubation with rapamycin (200 ng/mL) has in both, wild type and ptc1 cells. It has been shown that inhibition of the TOR pathway by treatment with rapamycin has profound effects on the global transcriptional profile. We confirmed the previously described transcription changes induced by the drug in wild type cells. Under our working conditions rapamycin increased, in wild type cells, at least 2-fold the expression of 667 genes, whereas it decreased the mRNA level of 721 genes (13.8% and 14.9% of genes with measurable expression level, respectively). Gene ontology analysis shows that, as previously documented, many induced genes falls into the NCR, Msn2/Msn4 regulated stress response or Retrograde response categories, whereas genes encoding cytoplasmic, but not mitochondrial, ribosomal proteins and the so called RIBI regulon where largely repressed. Deletion of PTC1 decreases the number of genes induced or repressed at least 2-fold by rapamycin treatment. Remarkably, lack of Ptc1 seems to lead to a general attenuation of changes triggered by rapamycin. The wt and the ptc1 mutant strains were analyzed in this series. We compared the expression profile of each strain treated with Rapamycin (200 ng/ml for 1h) with that of the same strain mock-treated (90% ethanol, 10% Tween-20). A Dye-swap was carried out for each RNA sample. Total number of chips analyzed: 4

2008-12-31 | E-GEOD-11530 | biostudies-arrayexpress

Barley host response to the direct application of the trichothecene mycotoxin deoxynivalenol

Project description:Barley florets (cv. Morex) were treated with 2.0 microgram deoxynivalenol per floret via a 10 microliter solution or mock inoculated with water. Samples were collected at 1, 12, 24, and 48 hours after inoculation. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Stephanie A. Gardiner. The equivalent experiment is BB62 at PLEXdb.] time: 1 hai - treated or untreated: Deoxynivalenol(3-replications); time: 1 hai - treated or untreated: Water(3-replications); time: 12 hai - treated or untreated: Deoxynivalenol(3-replications); time: 12 hai - treated or untreated: Water(3-replications); time: 24 hai - treated or untreated: Deoxynivalenol(3-replications); time: 24 hai - treated or untreated: Water(3-replications); time: 48 hai - treated or untreated: Deoxynivalenol(3-replications); time: 48 hai - treated or untreated: Water(3-replications)

2011-11-22 | E-GEOD-33405 | biostudies-arrayexpress

The effect of Fusarium culmorum infection and deoxynivalenol (DON) application on proteome response in barley cultivars Chevron and Pedant

Project description:The effect of an artificial infection with Fusarium culmorum and an application of deoxynivalenol (DON) on barley spikes of cultivars Chevron and Pedant during flowering was investigated at grain mid-dough stage 10 days after pathogen inoculation (10 dai). Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with LC-MS/MS was used to investigate the quantitative or qualitative differences between the experimental variants.

2017-09-07 | PXD006432 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data