Mammalian microRNAs: Experimental evaluation of novel and previously annotated genes

ABSTRACT: MicroRNAs (miRNAs) are small regulatory RNAs that derive from distinctive hairpin transcripts. To learn more about the miRNAs of mammals, we sequenced 60 million small RNAs from mouse brain, ovary, testes, embryonic stem cells, three embryonic stages, and whole newborns. Analysis of these sequences confirmed 387 annotated miRNA genes and identified 110 novel miRNA genes. Over 150 previously annotated miRNAs and hundreds of candidates failed to yield sequenced RNAs with miRNA-like features. Ectopically expressing these previously proposed miRNA hairpins also did not yield small RNAs, whereas ectopically expressing the confirmed and newly identified hairpins usually did yield small RNAs with the classical miRNA features, including dependence on the Drosha endonuclease for processing. These e

ORGANISM(S): Homo sapiens

SUBMITTER: H. Rosaria Chiang 

PROVIDER: E-GEOD-20384 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress