Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparison of GFP- and Nurr1-infected ES-cell derived neurons

ABSTRACT: ES cell-derived neurons of forebrain identity were isolated by magnetic sorting, cultured for 7 days and transduced with either Nurr1 or eGFP lentivirus. After an additional 12 h in culture, mRNA was isolated and subjected to microarray analysis. The raw data have been provided in CEL format The processed data have been provided in CHP format ES-cell derived forebrain neurons were plated in 10cm dishes and infected with either GFP or Nurr1 lentivirus at a MOI of 10. 18h after infection, RNA was prepped by the Qiagen mini kit and submitted to labeling and hybridization. Microarray experiments were carried out using GeneChip 430A 2.0 arrays (Affymetrix). Data was normalized and expression values were computed using the gcrma method. Statistical analysis was performed by empirical Bayes inference for linear models using the limma package. The inclusion criteria fold-change was > 1.6 and P < 0.001

ORGANISM(S): Mus musculus

DISEASE(S): Nurr1 lentivirus

SUBMITTER: Jessica Silvaggi 

PROVIDER: E-GEOD-20392 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

NR4A orphan nuclear receptors as mediators of CREB-dependent neuroprotection.

Volakakis Nikolaos N   Kadkhodaei Banafsheh B   Joodmardi Eliza E   Wallis Karin K   Panman Lia L   Silvaggi Jessica J   Spiegelman Bruce M BM   Perlmann Thomas T  

Proceedings of the National Academy of Sciences of the United States of America 20100621 27

Induced expression of neuroprotective genes is essential for maintaining neuronal integrity after stressful insults to the brain. Here we show that NR4A nuclear orphan receptors are induced after excitotoxic and oxidative stress in neurons, up-regulate neuroprotective genes, and increase neuronal survival. Moreover, we show that NR4A proteins are induced by cAMP response element binding protein (CREB) in neurons exposed to stressful insults and that they function as mediators of CREB-induced neu  ...[more]

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