Custom microchip analysis identifies differentially expressed non-coding RNAs in Epstein-Barr virus-infected B cells

ABSTRACT: Upon Epstein-Barr virus (EBV) infection of human B lymphocytes non-coding RNAs (ncRNAs) regulate expression of viral and cellular genes. In this study, we generated a specialized cDNA library from EBV-immortalized cells and subjected it to deep sequencing. We identified 631 unique ncRNA genes, comprised of 321 potential novel differentially expressed ncRNA candidates. Subsequently, we investigated differential expression of known and potential novel ncRNA candidates by custom-designed microchips by comparing expression of ncRNA genes of EBV-immortalized versus non-infected control cells. Among the differentially expressed candidates from chip analysis, differential expression of six novel ncRNA candidates was verified by northern blot analysis. In addition, microchip analysis resulted in o

ORGANISM(S): Homo sapiens

SUBMITTER: Sonja Vorwerk 

PROVIDER: E-GEOD-20441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress