A novel way for enriching human melanoma precursor cells to identify cellular targets for antineoplastic therapy

ABSTRACT: Metastatic melanoma is difficult to treat and is resistant to most current therapies. We hypothesized that stem cell related pathways may play a role in melanoma growth since stem cells and cancer cells share essential properties. Lately, the ABC transporter, ABCB5, as well as Cripto-1, CD20, CD133 and ABCG2 were used to isolate tumorigenic cells. Melanoma cells expressing MDR1 (ABCB1) were found in our lab to be enriched with ABCB5, Nanog, and Oct3/4 expression and were characterized by increased self-renewal capacity and anchorage independence. Melanoma significant heterogeneity was further demonstrated by Quintana et al. who found high prevalence of melanoma initiating cells when implanted into severely immunocompromised mice. Therefore, we took a more general approach in order to unr

ORGANISM(S): Homo sapiens

SUBMITTER: jasmine jacob 

PROVIDER: E-GEOD-20469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress