Gene expression in hepatocytes and bile ducts from ANIT-treated rats

ABSTRACT: Acute toxic responses to a single 50 mg/kg oral dose of 1-napthylisothiocyanate (ANIT) were evaluated by microarray analysis of laser capture-microdissected rat biliary epithelium or hepatic parenchyma at 2 and 6 hours post-dose. Selective and dramatic differences between the biliary epithelium and the hepatic parenchyma were evident as soon as 2 hours at which time 375 genes were altered in biliary epithelium, but only a nominal 38 genes were altered in the hepatic parenchyma. Gene expression analysis revealed increased expression of genes involved in the unfolded protein response and ER stress in biliary epithelial cells, but no alteration of these genes in hepatocytes. By 6 hours post dose, 620 genes showed altered expression in biliary epithelium while there were only 32 genes altered

ORGANISM(S): Rattus norvegicus

SUBMITTER: John Cullen 

PROVIDER: E-GEOD-20498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress