Transcription profiling of human multiple myeloma cells co-cultured with bone marrow stromal cells in vitro

ABSTRACT: Conventional anti-cancer drug screening is typically performed in the absence of accessory cells (e.g. stromal cells) of the tumor microenvironment, which can profoundly alter anti-tumor drug activity. To address this major limitation, we have developed assays (e.g. the tumor cell-specific in vitro bioluminescence imaging (CS-BLI) assay) to selectively quantify tumor cell viability, in presence vs. absence of non-malignant stromal cells or drug treatment. These assays have allowed us to identify that neoplastic cells from diverse malignancies exhibit stroma-induced resistance to different anti-tumor agents. In this analysis, we evaluated the molecular changes triggered in myeloma cells by their in vitro interaction with stromal cells. The transcriptional profile of 3 human multiple myeloma

ORGANISM(S): Homo sapiens

SUBMITTER: Douglas McMillin 

PROVIDER: E-GEOD-20540 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress