Global analysis of gene expression by SV40 T antigen in mouse embryo fibroblasts

ABSTRACT: SV40 large T antigen (TAg) contributes to cell transformation, in part, by targeting two well characterized tumor suppressors, pRb and p53. TAg expression affects the transcriptional circuits controlled by Rb and by p53. We have performed a microarray analysis to examine the global change in gene expression induced by wild-type TAg and TAg-mutants, in an effort to link changes in gene expression to specific transforming functions. For this analysis we have used MEFs expressing TAg or infected by SV40. Our analysis indicates that TAg can induce interferon-stimulated genes in MEFs and that this induction depends upon the LXCXE motif and p53 binding. Two independent wild-type MEFs and three independent MEF cell lines expressing TAg and the mutants were used. Additionally, three independent mo

ORGANISM(S): Mus musculus

SUBMITTER: Paul Cantalupo 

PROVIDER: E-GEOD-20620 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress