Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide mapping of OCT4, NANOG and CTCF in human embryonic stem cells

ABSTRACT: Transcription factors and their specific interactions with targets are crucial in specifying gene expression programs. To gain insights into the transcriptional regulatory networks in embryonic stem cells, we used chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing (ChIP-seq) to map the locations of OCT4, NANOG and CTCF. ChIP-enriched DNA from pooled ChIP samples were analyzed by Solexa sequencing.

ORGANISM(S): Homo sapiens

SUBMITTER: Justin Joseph Gnanakkan 

PROVIDER: E-GEOD-20650 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transposable elements have rewired the core regulatory network of human embryonic stem cells.

Kunarso Galih G   Chia Na-Yu NY   Jeyakani Justin J   Hwang Catalina C   Lu Xinyi X   Chan Yun-Shen YS   Ng Huck-Hui HH   Bourque Guillaume G  

Nature genetics 20100606 7

Detection of new genomic control elements is critical in understanding transcriptional regulatory networks in their entirety. We studied the genome-wide binding locations of three key regulatory proteins (POU5F1, also known as OCT4; NANOG; and CTCF) in human and mouse embryonic stem cells. In contrast to CTCF, we found that the binding profiles of OCT4 and NANOG are markedly different, with only approximately 5% of the regions being homologously occupied. We show that transposable elements contr  ...[more]

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