Gene expression in human umbilical cord endothelial cells following premiR-100 overexpression

ABSTRACT: To search for genes regulated by microRNA-100 in endothelial cells, we transfected human umbilical cord endothelial cells (HUVECs) with miR-100 precursor oligonucelotides. Human umbilical vein endothelial cells (HUVECs) were isolated from donated umbilical cords, pooled from two donors and cultivated up to passage 5. For transfection with pre-miR microRNA precursor molecules cells were cultured to 70% confluence and transfected with 8nM pre-miR-100 or an irrelevant control oligonucleotide (both from Ambion) using Lipofectamin RNAiMax (Invitrogen) according to the manufacturers instructions. Total RNA was isolated 48h after transfection.

ORGANISM(S): Homo sapiens

SUBMITTER: Sebastian Grundmann 

PROVIDER: E-GEOD-20668 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress