Gene Expression in Gold Nanoparticle Oligonucleotide Complexes treated Primary Immune Cells

ABSTRACT: Gold nanoparticle (Au—NP) oligonucleotide complexes hold considerable promise as an approach for manipulating intracellular gene regulation. We show that the uptake and intracellular fate of Au—NP oligonucleotide complexes is associated with endocytosis and signal transduction. A transcriptome—wide functional analysis of gene expression implicated multiple signaling pathways specific for Au—NP oligonucleotide complexes. In primary immune cells, the complexes trigger the expression of pro—inflammatory non—chemotactic cytokines rather than the many IFN—stimulated genes critical for induction of the immune response to a microbial challenge. Concordant with these changes, exposure to Au—NP oligonucleotide complexes is also accompanied by marked activation of immune cells. This

ORGANISM(S): Homo sapiens

SUBMITTER: Eunyoung Kim 

PROVIDER: E-GEOD-20677 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress