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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

ABSTRACT: mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA microRNA mir-517a (MIR517A) was transfected to Huh-7 cells using GFP-expressing lentiviral vector. Infected cells were selected using flow cytometry and subjected to mRNA expression microarray experiment. The profiles were compared to controls cells infected with only GFP protein.

ORGANISM(S): Homo sapiens

SUBMITTER: Yujin Hoshida

PROVIDER: E-GEOD-20679 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA-based classification of hepatocellular carcinoma and oncogenic role of miR-517a.

Toffanin Sara S Hoshida Yujin Y Lachenmayer Anja A Villanueva Augusto A Cabellos Laia L Minguez Beatriz B Savic Radoslav R Ward Stephen C SC Thung Swan S Chiang Derek Y DY Alsinet Clara C Tovar Victoria V Roayaie Sasan S Schwartz Myron M Bruix Jordi J Waxman Samuel S Friedman Scott L SL Golub Todd T Mazzaferro Vincenzo V Llovet Josep M JM

Gastroenterology 20110213 5

<h4>Background & aims</h4>Hepatocellular carcinoma (HCC) is a heterogeneous tumor that develops via activation of multiple pathways and molecular alterations. It has been a challenge to identify molecular classes of HCC and design treatment strategies for each specific subtype. MicroRNAs (miRNAs) are involved in HCC pathogenesis, and their expression profiles have been used to classify cancers. We analyzed miRNA expression in human HCC samples to identify molecular subclasses and oncogenic miRNA ...[more]

PMID: 21324318

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MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a

Project description:Hepatocellular carcinoma (HCC) is a complex and heterogeneous tumor due to activation of multiple cellular pathways and molecular alterations. Herein, we report the first molecular classification of 89 HCC based on the expression of 358 microRNAs and integrative genomic analysis. Three main subclasses of HCC were identified : two of them were associated with beta-catenin mutations or aggressive phenotype. A subset of the subclass of aggressive tumors (8/89, 9%) showed overexpression of a cluster of microRNAs located on chr19q13.41 (C19MC locus. We showed that miR 517a, representing C19MC, promoted cell proliferation, migration and invasion in vitro and induced the development of aggressive tumors in vivo suggesting its role as a novel oncogenic driver in HCC. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2011-02-17 | E-GEOD-20596 | biostudies-arrayexpress

mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

Project description:mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA

2011-02-17 | GSE20679 | GEO

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microRNA profiling of human healthy liver and hepatocellular carcinoma

Project description:microRNA profiling of 10 human healthy liver and 9 human hepatocellular carcinoma tissues using bead-based technology microRNA expression profiling was performed using freshly frozen healthy liver and hepatocellular carcinoma tissues obtained at the time of surgical treatment. keywords: healthy liver, hepatocellular carcinoma, expression array, microRNA

2012-07-21 | E-GEOD-31383 | biostudies-arrayexpress

Expression data from Huh-7 cells

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A Daphnane Diterpenoid Isolated from Wikstroemia polyantha induces an inflammatory response and modulates miRNA activity

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2012-06-28 | E-GEOD-39002 | biostudies-arrayexpress

miR-122-dependent binding of TNRC6 to HCV RNA

Project description:The liver-specific microRNA, miR-122, is an essential host factor for replication of hepatitis C virus (HCV), an important infectious cause of chronic liver disease and hepatocellular carcinoma. miR-122 stabilizes the positive-strand HCV RNA genome and promotes viral RNA synthesis by binding two closely spaced sites (S1 and S2) near the 5’ end of the genome in association with Ago2. Ago2 is essential for both host factor activities, but whether other host proteins are involved is unknown. Using a quantitative proteomics approach, we identified TNRC6A (GW182) and its paralogs (TNRC6B and TNRC6C), as functionally important components of the miR-122/Ago2 host factor complex binding HCV RNA. Depletion of any two TNRC6 proteins reduced HCV replication in Huh-7.5 cells,but did not reduce viral RNA stability or translational activity, but rather dampened miR-122 stimulation of viral RNA synthesis. However, TNRC6 depletion had no effect on replication of HCV in which S2 was mutated so that miR-122 binds only S1, whereas it significantly enhanced replication when S1 was mutated and only S2 bound by miR-122. Consistent with this, we found that TNRC6 proteins preferentially associate with the S1 site, and that the association of Ago2 with S2 is increased in TNRC6-depleted cells. Collectively, these data suggest a model in which TNRC6 proteins, which are known to interact with Ago2, preferentially direct the miR-122/Ago2 complex to S1 while restricting its association with S2, thereby fine tuning the spatial organization of miR-122/Ago2 complexes bound to the viral RNA.

2019-04-24 | PXD013054 | Pride

Human hepatocarcinoma cells : Huh-7 vs. SNU-739

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Liver cancer progression through increased miR-221 mediated tumorigenesis

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