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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide expression analysis of mouse chromosome 2 in gonadal fat and liver tissues reveals a complex architecture of obesity

ABSTRACT: Transcript abundance in adipose and liver tissues was used to map expression quantitative trait loci (eQTL) underlying fat accretion, weight gain and liver weight. Gonadal fat and liver tissues were used from 48 individuals F2 selected from a cohort (1,200 mice) originated from a cross between M16i and MB2 mice. A complex polygenic architecture within the MB2 locus was observed which was comprised of several locally regulated eQTL (cis-eQTL) with significant correlations to obesity traits. Total RNA obtained from isolated gonadal fat and liver tissues were used to mesured transcript abundance in order to detect expression QTLs.

ORGANISM(S): Mus musculus

SUBMITTER: Gloria Muñoz

PROVIDER: E-GEOD-20724 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide expression analysis of mouse chromosome 2 in gonadal fat and liver tissues reveals a complex architecture of obesity

Project description:Transcript abundance in adipose and liver tissues was used to map expression quantitative trait loci (eQTL) underlying fat accretion, weight gain and liver weight. Gonadal fat and liver tissues were used from 48 individuals F2 selected from a cohort (1,200 mice) originated from a cross between M16i and MB2 mice. A complex polygenic architecture within the MB2 locus was observed which was comprised of several locally regulated eQTL (cis-eQTL) with significant correlations to obesity traits.

2010-09-15 | GSE20724 | GEO

adipose tissue gene expression profile from male WT and fat-1 transgenic 14 week old high fat fed mice

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2022-01-26 | PXD021046 | Pride

Multi-tissue expression profiling of TALLYHO/Jng and C57BL/6J Mice under high-fat and chow diets

Project description:In order to identify gene expression patterns that vary with diet, genetics, and their interaction, we performed expression profiling by RNA-Seq across four different tissues (liver, gonadal fat, soleus muscle, and pancreas) of TALLYHO/Jng (TH) and C57BL/6J (B6) male (M) and female (F) mice, fed either a chow or high fat (HF) diet at 5 weeks and 20 weeks of age.

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Project description:Analysis of tissues of DBA/2 mice fed a standard breeding diet (SBD) and high fat diet (HFD) revealed tissue specific roles in inflammation and disease, and altered communication between tissues. The tissues surveyed incuded adipose tissues (brown, inguinal, mesenteric, retro-peritoneal, subcutaneious and gonadal), muscle and liver. After the 6 weeks feeding period with different diets, individual blood and tissues were collected from 12 weeks old mice for the determination of serum factors, gene expression analyses and the determination of fatty acid profiles. Mice were individually fasted for two hours prior to dissection that was carried out between 1 PM and 3 PM on three successive days. After the fasting period, blood was withdrawn from the retroorbital plexus of each mouse through a heparin-coated hematocrit tube into a 1.5 ml tube and placed on room temperature until all samples were centrifuged at 600 x g for 10-15 min to obtain serum for the analysis of lipids, glucose, insulin and leptin. Subsequently, animals were sacrificed by cervical dislocation. After bleeding, the subcutaneous fat pads (mainly inguinal fat pads), the gluteal fat pads (between legs left and right to the after), the quadriceps (musculus rectus femulus, m. vastus intermedius, m. vastus lateralis, m. vastus medialis), the gonadal fat pads (surrounding gonads), the retroperitoneal fat pads (below the kidney), the liver, the mesenteric fat pad (hanging at the intestine), and the brown adipose tissues (surrounded by white adipose tissue) were carefully dissected in the given order and immediately collected in RNAlater (Ambion, Austin, TX). The sampling protocol lasted no longer than 15 min per animal.

2010-04-13 | E-GEOD-15822 | biostudies-arrayexpress

RNA-seq analysis of tissues from PKCepsilon defiient mice

Project description:Comparison of RNA expression in liver and gonadal fat from female adipose-specific protein kinase C epsilon knockout mice and littermate controls, fed a high fat diet

2018-10-11 | GSE109835 | GEO

DNA methylomes and RNA transcriptomes in 19-weeks-old CD-1 male epididymal white adipose tissues with or without prenatal exposure to 500 ug/kg/day Bisphenol A

Project description:Exposure of mammalian fetuses to endocrine disruptors can increase the risk of adult-onset diseases. For example, we previously showed that exposure of mouse fetuses to bisphenol A (BPA) caused adult-onset obesity. To obtain insights into roles of epigenetic changes in the delayed toxic effects of endocrine disruption, we determined effects of fetal mouse exposure to BPA on genome-wide DNA methylation and mRNA expression in gonadal white adipose tissues by deep sequencing (MBD-seq and RNA-seq), bisulfite pyrosequencing, and real-time qPCR. Pregnant CD-1 mice (F0) were dosed daily with 0, 5, or 500 ug/kg/day BPA during 9-18 dpc, and the weaned F1 animals were fed ad libitum with standard chow until they were sacrificed at 19 weeks old. In the vehicle-exposed F1 animals, fggy promoter showed a clear bimodal pattern of very strong (55-95%) or very weak (5-30%) DNA methylation occurring at nearly equal incidence with no intermediate strength, and promoter hypermethylation completely suppressed mRNA expression. BPA exposure eliminated this naturally occurring dichotomy, shifting fggy promoter towards the hypomethylation state to release transcriptional suppression. Strength of Fggy mRNA expression significantly correlated with increased whole-body weight and gonadal fat weight of males but not females. Bioinformatics studies showed that expression of Fggy mRNA is stronger in mouse white adipose tissues than brown adipose tissues and enhanced in gonadal fat by diet-induced obesity. These observations suggest that prenatal exposure to BPA may disrupt the physiological bimodal nature of epigenetic regulation of fggy in mouse white adipose tissues, possibly contributing to the adult-onset obesity phenotype.

2018-08-27 | GSE107198 | GEO

Microarray gene expression data in liver, gonadal adipose tissue, pancreatic islets, and skeletal muscle in two lines of the Berlin Fat Mouse (BFMI861-S1 and BFMI861-S2)

Project description:The Berlin Fat Mouse Inbred line (BFMI) is a model for obesity and the metabolic syndrome. This study aimed to identify genetic variants associated with liver weight, liver triglycerides, and body weight using the obese BFMI sub-line BFMI861-S1. BFMI861-S1 mice are insulin resistant and store ectopic fat in the liver.

2022-03-27 | E-MTAB-11512 | biostudies-arrayexpress

Hepatic gene expression profiling reveals key pathways involved in leptin mediated weight loss in ob/ob mice.

Project description:The purpose of this study was to identify leptin target genes and subsequent pathways correlated with leptin-mediated weight loss. We utilized the microarray technology to compare two types of leptin administration: one involving a direct stimulatory effect when administered peripherally (subcutaneous: SQ) and another that is indirect, involving a hypothalamic relay that suppresses food intake when leptin is administered centrally (intracerebroventricular: ICV). We report here the impact of central and peripheral administration of leptin on food intake, body weight and body fat composition in ob/ob mice. We also report hepatic gene expression changes caused by central versus peripheral leptin administration. Keywords: comparison Leptin deficient (ob/ob) mice were continuously administered leptin over 12-days using central (intracerebroventricular) or peripheral (subcutaneous) route of administration. Liver RNA was extracted and hybridized to Illumina microarrays and gene expression data was analyzed. The global gene expression profiles were compared after the central and peripheral leptin treatments in ob/ob mice and C57BL6 mice were used for the baseline gene expression.

2010-11-10 | E-GEOD-20878 | biostudies-arrayexpress

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