Transcription profiling of mouse fibroblasts to understand the effect of radiation on Oct-1 deficient and wild type mouse strains.

ABSTRACT: The POU-domain transcription factor Oct-1 is widely expressed in adult tissues and has been proposed to regulate a large group of target genes. Microarray expression profiling was used to evaluate gene expression changes in Oct-1-deficient mouse fibroblasts. A number of genes associated with cellular stress exhibited altered expression. Consistent with this finding, Oct-1-deficient fibroblasts were hypersensitive to radiation, doxorubicin, and hydrogen peroxide and harbored elevated reactive oxygen species. Expression profiling identified a second group of genes dysregulated in Oct-1-deficient fibroblasts following irradiation, including many associated with oxidative and metabolic stress. A number of these genes contain octamer sequences in their immediate 5' regulatory regions, some of

ORGANISM(S): Mus musculus

SUBMITTER: Sanchita Bhattacharya 

PROVIDER: E-GEOD-2084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress