Gene expression profiles of PNT1a prostate cells expressing FGFR-4 Arg388 or Gly388.

ABSTRACT: We have shown that in PNT1a cells expressing exogenous FGFR4 Arg388 or Gly388 under the control of the EF1 promoter almost all FGFR signaling can be attributed to the transfected receptor, and the two FGFR-4 isoforms are expressed at equivalent levels in the two cell lines. Furthermore, we have shown that serum contains abundant FGFs capable of activating FGFR-4 so these initial experiments were carried out in serum to mimic physiological conditions. In such conditions the Arg388 expressing PNT1a cells display increased invasiveness and motility compared to Gly388 expressing cells. FGFR-4 Arg388 allele shows increased receptor stability and sustained receptor activation following ligand binding when compared to the Gly388 allele. However, the impact of this sustained signaling on cellular

ORGANISM(S): Homo sapiens

SUBMITTER: Chad Creighton 

PROVIDER: E-GEOD-20906 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress