Expression analysis of Shigella sonnei MS390 delta-rodZ mutant

ABSTRACT: Expression of type III secretion system (TTSS), a major determinant of virulence in Shigella, is markedly inhibited in a deletion mutant (cpxA) of the CpxAR two-component sensor at the level of post-transcriptional processing of the second TTSS regulator, InvE. A novel mutant of rodZ, which encodes a cytoskeletal protein involved in maintaining the rod-shaped morphology of bacilli, was isolated in a screen for mutations that restored TTSS expression in a cpxA mutant. The rodZ mutants of Shigella sonnei efficiently expressed InvE at 30°C, a temperature at which expression is normally repressed through temperature-dependent post-transcriptional regulation. Consistent with a marked increase in invE mRNA stability in the rodZ mutant, purified RodZ strongly bound to an invE RNA probe. Functiona

ORGANISM(S): Shigella sonnei

SUBMITTER: Jiro Mitobe 

PROVIDER: E-GEOD-21051 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress