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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis of HepaRG cells that ectopically express miR-371-3 cluster

ABSTRACT: the immoratlized hepatic cell line HepaRG was transduced with a retroviral vector containing the miR-371-3 cluster. As a control, transduction was performed with the empty vector. Transcriptome analysis was performed by using the Affymetrix whole-transcript human gene 1.0 ST array. Each condition was analyzed in triplicate hierarchical unsupervised clustering of HB samples according to their miRNA expression profile identifies 2 tumor subgroups associated with different degree of differentiation, proliferation rate and invasive phenotype. As subset of these tumors is used as training set to build a mir classifier. When the signature is applied to an independent test set, an independent series of HB is classified patients into two groups associated with the clinical features identified in the first set of patients.

ORGANISM(S): Homo sapiens

SUBMITTER: stefano cairo

PROVIDER: E-GEOD-21080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis of HepaRG cells that ectopically express miR-371-3 cluster

Project description:the immoratlized hepatic cell line HepaRG was transduced with a retroviral vector containing the miR-371-3 cluster. As a control, transduction was performed with the empty vector. Transcriptome analysis was performed by using the Affymetrix whole-transcript human gene 1.0 ST array. Each condition was analyzed in triplicate

2012-12-31 | GSE21080 | GEO

Generation of functional cholangiocytes from human pluripotent stem cells and HepaRG cells

Project description:Gene expression profile analysis allowed to identify a panel of genes and pathways characteristic of hESC-and HepaRG-derived cholangiocytes. Microarrays were conducted at day 23 and day 10 of differentiation for hESC-Chol and HepaRG-Chol respectively and compared to hESC-HB and HepaRG-HB. Five, four or three independent experiments were performed depending the experimental sample

2013-10-28 | E-GEOD-51791 | biostudies-arrayexpress

Analysis of Hepatoblastoma and normal fetal liver miRNA profiles and HepaRG cells that ectopically express miR-371-3 cluster

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-13 | E-GEOD-21085 | biostudies-arrayexpress

Effects of Combined Persistant Organic Pollutants on Global Gene Expression in Human HepaRG Cells

Project description:The exposure to and contamination by Persistent Organic Pollutants (POPs), which include pesticides used worldwide and polyaromatic hydrocarbons, is detrimental to human health and diverse ecosystems. Although most mechanistic studies have focused on single compounds, living organisms are exposed to multiple environmental xenobiotics, simultaneously, throughout their lives. The experimental evidence useful for assessing the effects of exposure to pollutant mixtures is scarce. We investigated the effects of exposure to a combination of two POPs, which employ different xenosensors, on global gene expression in a human hepatocyte cell model, HepaRG. Whole genome microarrays were used to investigate the effects on the HepaRG transcriptome following exposure to the combination of POPs as compared to each compound individually. Differentiated HepaRG cells were treated with either 2,3,7,8 tetrachlorodibenzo-p-dioxin, alpha-endosulfan (an organochlorine pesticide), the mixture or the DMSO vehicle for 30 hours after which RNA was extracted for hybridization on Affymetrix whole human genome microarrays.

2016-07-03 | E-GEOD-46874 | biostudies-arrayexpress

Hepatocytes-HepaRG reprogramming.

2013-12-05 | GSE52989 | GEO

Untargeted metabolomics to study ethanol induced hepatotoxicity in HepaRG cells

Project description:HepaRG cells were exposed to ethanol at the IC10 (H) and 1/10 of the IC10 (L) for 24 h and 48 h. Control samples and extraction blanks are denoted by C and B, respectively. The original experiment was validated using a second batch of HepaRG cells with the same experimental set-up.

2022-01-04 | MSV000088638 | MassIVE

DMSO effect on HepaRG differentiation

Project description:Gene expression profile analysis allowed to identify a panel of genes characteristic of HepaRG differentiation and DMSO effect on the differentiation process.

2022-09-06 | GSE112123 | GEO

Hepatocytes-HepaRG reprogramming.

Project description:To identify the molecular mechanisms and environmental inducers contributing to reprogramming of hepatocytes into progenitors in HCC context, we used the HepaRG cell line as model. We performed transcriptomic analysis at various time-points during this process; 0h, 1h, 4h, 8h, 12h, 16h, 24h and 48h.

2013-12-05 | E-GEOD-52989 | biostudies-arrayexpress

Analysis of Hepatoblastoma and normal fetal liver miRNA profiles and HepaRG cells that ectopically express miR-371-3 cluster

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-12-13 | GSE21085 | GEO

Proteome and secretome of HepaRG cells

Project description:Primary human hepatocytes, the gold standard for in vitro studies of liver-related functions, suffer from uncertain availability and high variability in cell activity. Over years, a number of alternative hepatic cell lines have lost major liver-like functions, but not HepaRG cells. Therefore, their increasing use worldwide today arouses the need for establishing a reference functional status of differentiated HepaRG cells known as HPR116, which originate from the initial cell bank. Deep proteome and secretome analyses enabled us to show that they nicely express, at levels generally close to those in primary hepatocytes, master regulators of the hepatic phenotype, structural elements that ensure liver-like polarisation and factors supporting their transdifferentiation properties. Their highly differentiated status, mitochondrial functionality, hepatokine secretion ability and response to insulin was verified. Overall, the HepaRG cell system appears as robust surrogate cell system to primary hepatocytes, versatile enough to study not only xenobiotic detoxification but also the control of hepatic energy metabolism, secretory function and disease-related signalling pathways.

2019-02-27 | PXD010304 | Pride

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