Rainbow trout red blood cells (RBCs): Control vs Heat shock, repeated samples from individuals

ABSTRACT: The retention of a nucleus in the mature state of fish red blood cells (RBCs), and the ability to easily collect and manipulate blood in non-terminal experiments, makes it an ideal tissue on which to study the cellular stress response in fish. Through the use of the cGRASP 16K salmonid microarray, we are currently investigating differences in RBC global gene transcription in fish held under control conditions (12C) and exposed to heat stress (one hour at 25C followed by recovery at 12C). Repeated blood sampling (via a dorsal aorta cannula) enables us to examine the individual stress response over time. Samples were taken pre-heat stress (representing individual control) and at 4 and 24 hours post heat stress (representing early and late transcriptional regulation). A total of ~3000 feature

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Johanne Lewis 

PROVIDER: E-GEOD-21084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress