Expression of constitutively active FOXO3 in murine forebrain leads to a loss of neural progenitors

ABSTRACT: We have generated transgenic mice with tetracycline-regulated conditional expression of a constitutively active allele of FoxO3 under the control of the forebrain-specific CaMKIIa promoter. In adult animals, there was a reduction of brain weight by 30% and an almost complete loss of the dorsal dentate gyrus with normal cortical layering. Interestingly, the adult mice showed motor hyperactivity and a selective loss of long-term memory with normal spatial learning. We observed enhanced apoptosis starting from day E10.5. Performing microarray expression analyses and Q-PCR validation with E12.5 forebrain RNA, we observed an over-representation of thalamic markers and an under-representation of cortical markers in transgenic as compared to control animals. Immunohistochemical data show a loss o

ORGANISM(S): Mus musculus

SUBMITTER: Karlheinz Holzmann 

PROVIDER: E-GEOD-21089 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress