Microarray Profile of Gene Expression during Osteoclast Differentiation in Modeled Microgravity

ABSTRACT: Microgravity leads to a 10-15% loss of bone mass in astronauts during space flight. Osteoclast is the multinucleated bone resorbing cell. In this study, we used NASA developed ground based Rotary Wall Vessel Bioreactor (RWV), Rotary Cell Culture System (RCCS) to simulate microgravity (μXg) conditions and demonstrated a significant increase (2-fold) in osteoclastogenesis compared to ground based control (Xg) mouse bone marrow cultures. We further determined the gene expression profiling of RAW 264.7 osteoclast progenitor cells in microgravity by agilent microarray analysis. Gene expression pattern was functional group clustered by transcriptome analysis using gene ontology tree machine (GOTM) for cell proliferation/survival, differentiation and function. We confirm the microgravity modulat

ORGANISM(S): Mus musculus

SUBMITTER: Sambandam Yuvaraj 

PROVIDER: E-GEOD-21093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress