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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Massive decrease of maternal polyA mRNA upon post-ovulatory aging in Xenopus tropicalis

ABSTRACT: We used microarrays to detail the global gene expression changes following aging of oocytes We compared fresh oocytes with post-ovulatory aged oocytes by microarray analysis

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Ludger Klein-Hitpass

PROVIDER: E-GEOD-21109 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Aging of Xenopus tropicalis eggs leads to deadenylation of a specific set of maternal mRNAs and loss of developmental potential.

Kosubek Anna A Klein-Hitpass Ludger L Rademacher Katrin K Horsthemke Bernhard B Ryffel Gerhart U GU

PloS one 20101022 10

As first shown more than 100 years ago, fertilization of an aged (overripe) egg increases the rate of malformations and embryonic loss in several vertebrates, including possibly humans as well. Since the molecular events in aging eggs may be similar in these species, we established in the frog Xenopus tropicalis a defined protocol for delayed fertilization of eggs. A three-hour delayed fertilization led to a dramatic increase in malformation and mortality. Gene expression profiling revealed that ...[more]

PMID: 21042572

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Massive decrease of maternal polyA mRNA upon post-ovulatory aging in Xenopus tropicalis

Project description:We used microarrays to detail the global gene expression changes following aging of oocytes

2011-03-29 | GSE21109 | GEO

High-throughput sequencing of small RNAs from Xenopus tropicalis

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Proteogenomics of Candida tropicalis – an opportunistic pathogen

Project description:Candida tropicalis is an opportunistic pathogen which causes candidiasis in immune-compromised individuals. It is one of the members of the non-albicans group of Candida that are known to be azole resistant and is frequently seen in individuals being treated for cancers, HIV-infection and bone-marrow transplant. Although the genome of C. tropicalis was sequenced in the year 2009, the genome annotation has not been supported by experimental validation. In the present study, we have carried out in-depth proteomic profiling of C. tropicalis using high-resolution Fourier transform mass spectrometry and mapped ~44% of the computationally predicted protein-coding genes with peptide level evidence. In addition to identifying 2,740 proteins in the cell lysate of this yeast, we also analysed the proteome of the conditioned media of C. tropicalis culture and identified several unique secreted proteins among a total of 780 proteins. By subjecting the mass spectrometry data derived from cell lysate and conditioned media to proteogenomic analysis, we identified 86 novel genes, 12 novel exons and corrected 49 computationally predicted gene models. To our knowledge, this is the first high-throughput proteomic study to refine the genome annotation of C. tropicalis.

2017-05-08 | PXD001071 | Pride

Gene expression changes induced in the stromal cell line EL08-1D2 by co-culture with leukemic B cells (CLL)

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Effect of NF-kappaB activation in bone marrow stromal cells co-cultured with CLL cells

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Stable intronic sequence RNA (sisRNA), a new class of noncoding RNA from the oocyte nucleus of Xenopus tropicalis

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Protein kinase C-beta dependent activation of NF-kB in stromal cells is indispensable for the survival of chronic lymphocytic leukemia in B-cells in vivo.

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-03-08 | E-GEOD-36416 | biostudies-arrayexpress

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