Chronological Aging of Yeast in the Absence of Caloric Restriction: Cell Immobilization Uncouples Reproduction from Metabolism

ABSTRACT: Studies using yeast have advanced our understanding of both replicative and chronological aging, leading to the discovery of longevity genes that have homologues in higher eukaryotes. Chronological lifespan in yeast is conventionally defined as the lifespan of a non-dividing cell. To date, this parameter has only been estimated under calorically restricted (CR) conditions, mimicked by starvation. Since post-mitotic cells in higher eukaryotes are rarely calorically-restricted, we sought to develop an alternative experimental system where non-dividing yeast would age chronologically, in the presence of excess nutrients. We report here on a system wherein alginate-encapsulated yeast are packed in a pH- and temperature-controlled bioreactor, then continuously fed non-limiting substrate for ext

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Kate McInnerney 

PROVIDER: E-GEOD-21187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress