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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of the ethanol tolerant strain HG228 in comparison to its parental strain Escherichia coli MG1655

ABSTRACT: The goal of this experiment is to identify the pathways that are deregulated as part of an adaptive response to high levels of ethanol in the media. Ethanol tolerant cells (HG228) grown in minimal media plus 1.5% (v/v) ethanol were hybridized against E. coli MG1655 grown under similar conditions. The experiment was done in duplicates using biological replicates.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Hani Goodarzi

PROVIDER: E-GEOD-21194 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulatory and metabolic rewiring during laboratory evolution of ethanol tolerance in E. coli.

Goodarzi Hani H Bennett Bryson D BD Amini Sasan S Reaves Marshall L ML Hottes Alison K AK Rabinowitz Joshua D JD Tavazoie Saeed S

Molecular systems biology 20100601

Understanding the genetic basis of adaptation is a central problem in biology. However, revealing the underlying molecular mechanisms has been challenging as changes in fitness may result from perturbations to many pathways, any of which may contribute relatively little. We have developed a combined experimental/computational framework to address this problem and used it to understand the genetic basis of ethanol tolerance in Escherichia coli. We used fitness profiling to measure the consequence ...[more]

PMID: 20531407

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