Chromatin and Sequence Features that Define the Fine and Gross Structure of Genomics Methylation Patterns

ABSTRACT: We report a new method for genome-wide methylation profiling that is able to probe methylation status in both single-copy DNA and interspersed repeats. This method, MethylMAPS, uses methylation-sensitive and -dependent enzymes to fractionate the genome according to methylation state. Methylated and unmethylated fragments are then sequenced with Next-Gen sequencing to map methylated and unmethylated CpG sites in the genome. We have used this method to determine the methylation status of >275 million CpG sites in human and mouse DNA from breast and brain tissues. We conclude that methylation is the default state of most CpG dinucleotides and that a combination of local dinucleotide frequencies, the interaction of repeated sequences, and the presence or absence of histone variants or modifi

ORGANISM(S): Mus musculus

SUBMITTER: John Edwards 

PROVIDER: E-GEOD-21242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress