Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression profiling of microRNAs in primary melanocytes

ABSTRACT: DICER is a central regulator of microRNA maturation. However little is known about mechanisms regulating its expression in development or disease. While profiling miRNA expression in differentiating melanocytes, two populations were observed: some upregulated at the pre-miRNA stage, and others upregulated as “mature” miRNAs (with stable pre-miRNA levels). Conversion of pre-miRNAs to fully processed miRNAs appeared to be dependent upon stimulation of DICER expression—an event found to occur via direct transcriptional targeting of DICER by the melanocyte master transcriptional regulator MITF. MITF binds and activates a conserved regulatory element upstream of DICER’s transcriptional start site upon melanocyte differentiation. Targeted knockout of DICER is lethal to melanocytes, at least partly via DICER-dependent processing of the pre-miRNA-17~92 cluster thus targeting BIM, a known pro-apoptotic regulator of melanocyte survival. These observations highlight a central mechanism underlying miRNA regulation which could exist for other cell types during development. Primary melanocytes were obtained from 7 independent donors. Mature-miRNA levels were detected using Applied Biosystem's TaqMan PCR assay.

ORGANISM(S): Homo sapiens

SUBMITTER: Jun Song

PROVIDER: E-GEOD-21249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Lineage-specific transcriptional regulation of DICER by MITF in melanocytes.

Levy Carmit C Khaled Mehdi M Robinson Kathleen C KC Veguilla Rosa A RA Chen Po-Hao PH Yokoyama Satoru S Makino Eiichi E Lu Jun J Larue Lionel L Beermann Friedrich F Chin Lynda L Bosenberg Marcus M Song Jun S JS Fisher David E DE

Cell 20100601 6

DICER is a central regulator of microRNA maturation. However, little is known about mechanisms regulating its expression in development or disease. While profiling miRNA expression in differentiating melanocytes, two populations were observed: some upregulated at the pre-miRNA stage, and others upregulated as mature miRNAs (with stable pre-miRNA levels). Conversion of pre-miRNAs to fully processed miRNAs appeared to be dependent upon stimulation of DICER expression--an event found to occur via d ...[more]

PMID: 20550935

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Dataset Information

Expression profiling of microRNAs in primary melanocytes

Publications

Lineage-specific transcriptional regulation of DICER by MITF in melanocytes.

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