Genome-wide screening of temporal responsive genes induced by a low concentration of the carcinogen N-methyl-N'-nitro-N-nitrosoguanidine in a normal human cell line

ABSTRACT: The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is a direct mutagen and carcinogen, causing DNA damage and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiation, and cell death. Our previous study revealed that MNNG at different concentrations could induce extensive changes in gene expression at an early stage of exposure. To further understand the dynamic cellular responses and hazardous effects caused by this environmental carcinogen, we used a whole-genome time-course screening methods to find out the gene expression changes induced by a low concentration of MNNG in human normal amnion epithelial FL cells. The cells were exposed to 1.0 M-BM-5M MNNG, and differential gene expression profiles at 3, 12, and 24 h after MNNG treatm

ORGANISM(S): Homo sapiens

SUBMITTER: li hongjuan 

PROVIDER: E-GEOD-21270 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress