Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

8 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression analysis of Thermus thermophilus HB8 wild-type strain in response to high growth temperature

ABSTRACT: In order to observe the heat-shock response of extremely thermohilic bacterium T. thermophilus HB8 strain, we analyzed the altered expression profile of the total mRNA in T. thermophilus HB8 wild-type strain after the growth temperature was shifted from 70Â°C to 80Â°C for 30 min. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70Â°C for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (0.5 mL) were inoculated into 250 mL of TT medium and then cultivated at 70Â°C for 6 hours. After the cells were collected from 50 ml culture medium, the remaining medium was cultured at 80Â°C for 30 min. The cells were collected, and then crude RNA was extracted from each lot of cells. The expression of each mRNA at each time point was analyzed on a GeneChip.

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Yoshihiro Agari

PROVIDER: E-GEOD-21288 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression profile of TTHA0175-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0175 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0175-deficient strain grown in a rich medium at 70Â°C for 300, 480, and 600 min. Two TTHA0175-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 300, 480, and 600 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21471 | biostudies-arrayexpress

Expression profile of TTHA1359-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1359 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the sdrP-deficient strain grown in a rich medium at 70Â°C for 300, 480, and 600 min. TTHA1359-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680, 1120, and 1240 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21472 | biostudies-arrayexpress

Expression profile of TTHA1438-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1438 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1438-deficient strain grown in a rich medium at 70Â°C for 240, 360, 480, 600 and 760 min. Two TTHA1438-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 240, 360, 480, 600, 680 and 760 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21198 | biostudies-arrayexpress

Expression profile of TTHB186-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHB186 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHB186-deficient strain grown in a rich medium at 70Â°C for 360, and 480 min. Three TTHB186-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 500 mL of the same medium and then cultivated at 70Â°C. Cells were collected after 360, and 480 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21457 | biostudies-arrayexpress

Expression profile of TTHA0252-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0252 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0252-deficient strain grown in a rich medium at 70Â°C for 360,540, and 680 min. Two TTHA0252-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 500 mL of the same medium and then cultivated at 70Â°C. Cells were collected after 360, 540, and 680 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21456 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium.

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TR) medium at 70Â°C. Keywords: time course Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TR medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2010-05-25 | E-GEOD-10368 | biostudies-arrayexpress

Expression profile of TTHA1431-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA1431 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA1431-deficient strain grown in a rich medium at 70Â°C for 360 min. Three TTHA1431-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1 L of the same medium and then cultivated at 70Â°C. Cells were collected after 360 min, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21462 | biostudies-arrayexpress

Expression profile of TTHA0845-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0845 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0845-deficient strain grown in a rich medium at 70Â°C for 420 min. Three TTHA0845-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1 L of the same medium and then cultivated at 70Â°C. Cells were collected after 420 min, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21461 | biostudies-arrayexpress

TTHB212 (anti-sigmaE)-deficient Thermus thermophilus HB8 strain: time course

Project description:We observed the expression profile of the total mRNA of TTHB212-deficient of Thermus thermophilus HB8 strain grown in a rich medium at 70Â°C for 7 and 8 hours TTHB212-deficient T. thermophilus HB8 cells were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 7 or 8 h, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-19758 | biostudies-arrayexpress

Expression profile of TTHA0973-deficient Thermus thermophilus HB8 strain

Project description:In order to find function of TTHA0973 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0973-deficient strain grown in a rich medium at 70Â°C for 680 min. Three TTHA0973-deficient T. thermophilus HB8 strains were each pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 680 min, respectively, and then, the expression of each mRNA was analyzed.

2010-10-15 | E-GEOD-21185 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data