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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Protein arginine methyltransferase AtPRMT5 is involved in pre-mRNA splicing in Arabidopsis thaliana

ABSTRACT: In this study, we analyzed the Arabidopsis homologue of PRMT5, AtPRMT5M-bM-^@M-^Ys function in RNA processing. RNA-seq analyses revealed that AtPRMT5 is involved in a subset of pre-mRNA splicing. Several RNA processing factors involved in regulating flowering time were validated that the corresponding intron retention surely exists in atprmt5 mutants. AtSm proteins can also be methylated by AtPRMT5 in vitro and in vivo, which may be the reasons for the pre-mRNA splicing defects in atprmt5. Contributed by The Institute of Genetics and Developmental Biology (IGDB) of the Chinese Academy of Sciences Investigate the role of AtPRMT5 in pre-mRNA splicing

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: xiaofeng cao

PROVIDER: E-GEOD-21323 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Arginine methylation mediated by the Arabidopsis homolog of PRMT5 is essential for proper pre-mRNA splicing.

Deng Xian X Gu Lianfeng L Liu Chunyan C Lu Tiancong T Lu Falong F Lu Zhike Z Cui Peng P Pei Yanxi Y Wang Baichen B Hu Songnian S Cao Xiaofeng X

Proceedings of the National Academy of Sciences of the United States of America 20101018 44

Protein arginine methylation, one of the most abundant and important posttranslational modifications, is involved in a multitude of biological processes in eukaryotes, such as transcriptional regulation and RNA processing. Symmetric arginine dimethylation is required for snRNP biogenesis and is assumed to be essential for pre-mRNA splicing; however, except for in vitro evidence, whether it affects splicing in vivo remains elusive. Mutation in an Arabidopsis symmetric arginine dimethyltransferase ...[more]

PMID: 20956294

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