Laser Capture Microdissection of Hyperlipidemic Mouse Aorta Atherosclerosis

ABSTRACT: Atherosclerosis is a transmural chronic inflammatory condition of small and large arteries that is associated with adaptive immune responses at all disease stages. However, impacts of adaptive immune reactions on clinically apparent atherosclerosis such as intima lesion (plaque) rupture, thrombosis, myocardial infarction, and aneurysm largely remain to be identified. It is increasingly recognized that leukocyte infiltrates in plaque, media, and adventitia are distinct but their specific roles have not been defined. To map these infiltrates, we employed laser capture microdissection (LCM) to isolate the three arterial wall laminae using apoE-/- mouse aorta as a model. RNA from LCM-separated tissues was extracted and large scale whole genome expression microarrays were prepared. We observed

ORGANISM(S): Mus musculus

SUBMITTER: Andreas Habenicht 

PROVIDER: E-GEOD-21419 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress