Genome wide screen reveals WNT11, a noncanonical WNT gene, as a target of ETS transcription factor ERG

ABSTRACT: ERG is a transcription factor that is involved in leukomogenesis and its mRNA overexpression has been associated with poor prognosis in a subset of patients with T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML). Herein, a genome-wide screen of ERG target genes was conducted by chromatin immunoprecipitation-on-chip (ChIP-chip) in Jurkat cells. 342 significant annotated regions were derived from ChIP-chip experiments. Seventeen candidate promoter regions resulted in at least two-fold enrichment by quantitative PCR. Notably, ERG potential targets included WNT signaling genes: WNT2, WNT9A, WNT11, CCND1, and FZD7. Functionally, expression of WNT11 was downregulated with siRNA ERG knockdown and substantially upregulated in a tet-on ERG-inducible assay in K562 cells. T

ORGANISM(S): Homo sapiens

SUBMITTER: Liliana Mochmann 

PROVIDER: E-GEOD-21495 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress