The metalloregulatory site in Streptococcus pneumoniae AdcR, a zinc-activated MarR-family repressor

ABSTRACT: Streptococcus pneumoniae D39 AdcR (adhesion competence repressor) is the first metal-sensing member of the MarR (multiple antibiotic resistance repressor) family to be characterized. Expression profiling of a ∆adcR strain grown in liquid culture under microaerobic conditions revealed that transcript amounts for 13 genes were up-regulated relative to the wild-type strain, among them adcR, adcCBA, encoding a high affinity ABC uptake system for zinc, and genes encoding cell-surface zinc-binding pneumococcal histidine triad (pht) and adcII (lmb, laminin binding) proteins. Down-regulated transcripts included those encoding two putative zinc-containing alcohol dehydrogenases. Bacterial strains were grown exponentially in rich (BHI) media at 37C and an atmosphere of 5% CO2 to OD620~0.2, and we

ORGANISM(S): Streptococcus pneumoniae

SUBMITTER: Darin Cowart 

PROVIDER: E-GEOD-21506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress