Large-scale temporal gene expression profiling during gonadal differentiation and early gametogenesis in rainbow-trout

ABSTRACT: Animals and samplings: Research involving animal experimentation has been approved by the author's institution (authorization number 35-14) and conforms to NIH guidelines. All-male and all-female rainbow trout populations were obtained from the INRA experimental fish farm (Drennec, France) using breeders from the Mirwart strain. At 55 days post-fertilization (55 dpf, i.e., the onset of the free swimming), a male and female batch of 1500 fry each were transferred in 0.3 m3 tanks with recirculating water conditions. They were held at 12°C under constant photoperiod (12h light : 12h dark) and fed ad libitum with a commercial diet (dry pellet food, BiomarTM, Brande, Denmark). In each group, from 20 to 100 gonads, depending on the age of the fish, were sampled and pooled in duplicates at variou

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Daniel Baron 

PROVIDER: E-GEOD-2151 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress